Effect of some substances produced in the pre-simbiontic phase on the morphology of Tuber borchii Vittad. mycelium
نویسنده
چکیده
Effects of 6 substances of 29 specifically expressed in the pre-simbiotic phase of Tuber borchii − Tilia americana interaction and root extracts obtained in water and ethanol were tested on T. borchii mycelium. Root extracts increased hyphal branching; this is in agree with data obtained using only endo-mycorhhizal fungi. The tested substances showed various effects on morphology and features of mycelia. Probably lipophylic substance is present in root extracts and causes an increase of hyphal branching, wheres the other substances, perhaps except benzothiazol, do not seem directly involved in the pre-simbiotic interaction.
منابع مشابه
Competitive PCR for quantitation of a Cytophaga-Flexibacter-Bacteroides phylum bacterium associated with the Tuber borchii Vittad. mycelium.
An uncultured bacterium associated with the ectomycorrhizal fungus Tuber borchii Vittad. was identified as a novel member of the Cytophaga-Flexibacter-Bacteroides group. Utilizing a quantitative PCR targeting the 16S rRNA gene, we relatively quantified this bacterium in the host. The estimated number of bacteria was found to be approximately 10(6) cells per 30-day-old T. borchii mycelium cultur...
متن کاملStudies on volatile organic compounds of Tuber borchii and T. asa-foetida.
Ascomata of two truffle species, Tuber borchii and T. asa-foetida, both naturally growing in woodlands of the Basilicata region (southern Italy), were identified on the basis of ascospore morphology and compared under a volatile organic compound profile to determine the particular volatile organic compounds that characterize each taxon. Solid-phase microextraction-gas chromatography-mass spectr...
متن کاملIsolation and characterization of differentially expressed genes in the mycelium and fruit body of Tuber borchii.
The transition from vegetative mycelium to fruit body in truffles requires differentiation processes which lead to edible fruit bodies (ascomata) consisting of different cell and tissue types. The identification of genes differentially expressed during these developmental processes can contribute greatly to a better understanding of truffle morphogenesis. A cDNA library was constructed from veg...
متن کاملChitin synthase homologs in three ectomycorrhizal truffles.
Degenerate PCR primers were used to amplify a conserved gene portion coding chitin synthase from genomic DNA of six species of ectomycorrhizal truffles. DNA was extracted from both hypogeous fruitbodies and in vitro growing mycelium of Tuber borchii. A single fragment of about 600 bp was amplified for each species. The amplification products from Tuber magnatum, T. borchii and T. ferrugineum we...
متن کاملTruffles regulate plant root morphogenesis via the production of auxin and ethylene.
Truffles are symbiotic fungi that form ectomycorrhizas with plant roots. Here we present evidence that at an early stage of the interaction, i.e. prior to physical contact, mycelia of the white truffle Tuber borchii and the black truffle Tuber melanopsorum induce alterations in root morphology of the host Cistus incanus and the nonhost Arabidopsis (Arabidopsis thaliana; i.e. primary root shorte...
متن کامل